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fluorescein conjugated avidin d  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein conjugated avidin d
    Fluorescein Conjugated Avidin D, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/A-2000-10/Avidin+D/pm42884857-327-5-8
    Average 96 stars, based on 1575 article reviews
    fluorescein conjugated avidin d - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries
    Article Snippet: .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA).

    Article Title: Intraspecific Karyotypic Differentiation in the Lycoris aurea Complex Revealed by Fluorochrome Banding and rDNA Fluorescence In Situ Hybridization.
    Article Snippet: .. Biotin-labeled probes were detected using fluorescein-conjugated avidin D (Vector Laboratories, Burlingame, CA, USA). ..

    Article Title: Pre-injury subchronic stress confers sex-specific protection against pain-associated symptoms in osteoarthritic mice
    Article Snippet: .. Finally, tissue was incubated for 5min with tyramide solution (Ape Bio, A8011-APE), washed 2 x 15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). ..

    Article Title: Voluntary wheel running provides pain relief but transiently exacerbates gait impairments in male and female mice with unilateral osteoarthritis
    Article Snippet: Next day, sections were washed in 0.1M PB then incubated for 2h with rabbit biotinylated secondary antibody (1:400; Bethyl Labs, A120-108B), followed by incubation in preformed avidin-biotin-HRP complexes (1:200; Vectastain ABCKit Peroxidase, PK-6100) for 30min. .. Finally, tissue was incubated for 5min with tyramide solution (ApeBio, A8011-APE), washed 2×15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). .. Sections were given a final wash, mounted on gelatinised slides, and coverslipped with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich, F4680).

    Article Title: SLC35A3 remodels site-specific N-glycosylation and defines a druggable glycosylation target in hepatocellular carcinoma.
    Article Snippet: After transfer to PVDF membranes, membranes were blocked with Carbo-Free Blocking Solution (Cat. No. SP-5040-125, Vector Laboratories, Newark, CA, USA) for 1 h at room temperature. .. Membranes were then incubated with biotinylated lectins (ConA, MAL-II, SNA, or AAL; Vector Laboratories) at room temperature for 30 min. After washing three times with TBST (5 min each), membranes were incubated with horseradish peroxidase-conjugated avidin D (Vector Laboratories) at room temperature for 30 min. ..

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries.
    Article Snippet: For the TmSat3 probe, considering TmSat3 monomer length of 1264 bp, we used four different primer pairs to bridge the entire monomer sequence, using the TmSat3 monomer fragment cloned into the plasmid vector pUC18 as a template. .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. The 500 bp TmSat2-specific fragment, cloned into the plasmid vector pUC18, was biotin-labeled by nick translation using the Nick Translation Kit (Roche, Switzerland).

    Amplification:

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries
    Article Snippet: .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA).

    Article Title: Tribolium madens satellitome reveals a network of highly abundant satellite DNAs in megabase-sized regions hallmarked by macro-dyad symmetries.
    Article Snippet: For the TmSat3 probe, considering TmSat3 monomer length of 1264 bp, we used four different primer pairs to bridge the entire monomer sequence, using the TmSat3 monomer fragment cloned into the plasmid vector pUC18 as a template. .. Cy3-labeled probes were detected directly after posthybridization washes, while detection of biotin-labeled probes was achieved using a fluorescein-conjugated avidin D (FITC-avidin D) and biotinylated anti-avidin D amplification system (Vector Laboratories, USA). .. The 500 bp TmSat2-specific fragment, cloned into the plasmid vector pUC18, was biotin-labeled by nick translation using the Nick Translation Kit (Roche, Switzerland).

    Incubation:

    Article Title: Pre-injury subchronic stress confers sex-specific protection against pain-associated symptoms in osteoarthritic mice
    Article Snippet: .. Finally, tissue was incubated for 5min with tyramide solution (Ape Bio, A8011-APE), washed 2 x 15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). ..

    Article Title: Voluntary wheel running provides pain relief but transiently exacerbates gait impairments in male and female mice with unilateral osteoarthritis
    Article Snippet: Next day, sections were washed in 0.1M PB then incubated for 2h with rabbit biotinylated secondary antibody (1:400; Bethyl Labs, A120-108B), followed by incubation in preformed avidin-biotin-HRP complexes (1:200; Vectastain ABCKit Peroxidase, PK-6100) for 30min. .. Finally, tissue was incubated for 5min with tyramide solution (ApeBio, A8011-APE), washed 2×15min with 0.1M PB, then incubated for an additional 2h with Avidin D, Fluorescein labelled (Vector Labs, A-2001-5). .. Sections were given a final wash, mounted on gelatinised slides, and coverslipped with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich, F4680).

    Article Title: SLC35A3 remodels site-specific N-glycosylation and defines a druggable glycosylation target in hepatocellular carcinoma.
    Article Snippet: After transfer to PVDF membranes, membranes were blocked with Carbo-Free Blocking Solution (Cat. No. SP-5040-125, Vector Laboratories, Newark, CA, USA) for 1 h at room temperature. .. Membranes were then incubated with biotinylated lectins (ConA, MAL-II, SNA, or AAL; Vector Laboratories) at room temperature for 30 min. After washing three times with TBST (5 min each), membranes were incubated with horseradish peroxidase-conjugated avidin D (Vector Laboratories) at room temperature for 30 min. ..



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